Technology Access Centre in Bio-Innovation (TAC-B)



La Cité

The TAC in Bio-Innovation supports Canadian businesses in realising applied research projects leading to the creation of new products and processes or the optimization of existing technology in the areas of industrial biotechnology, biopharmaceuticals and agri-food.

Environmental technology/biotechnology

Areas of Innovation Expertise

  • Agri-food
  • Analytical Chemistry
  • Bioinformatics
  • Bioprocesses
  • Biotechnology
  • Genetic Engineering

 

Point of Contact:

Christine Langlois, Msc.
Superviseure | Supervisor
Phone: 613-742-2483 x. 2980
Tél. Cell: 289-887-6060

Email: CLangl@lacitec.on.ca

Mailing address:

Centre d’accès à la technologie (CAT) en Bio-innovation
La Cité
801 Promenade de l’Aviation, Pièce B3210
Ottawa, Ontario
K1K 4R3

 

Technical and Business Services

Facilitation service (introduction)

Literature reviews

Problem solving

Product testing

Proof of concept

Advanced welding support for product development

Assistance with building products approvals and commercialization

Business Mentorship referrals

Early-Stage Business Development

Scientific report writing

 

Applied Research and Development

Coaching

Concept validation

Design of Experiment

Process Optimization

Product Re-design & Improvement

Laboratory/pilot scale prototyping

Manufacturing process design and development

Pre-clinical development (chemistry & analytical biochemistry, metabolomics, pharmaceutical science, cell biology & microbiology)

Product Design & Development

Technology Validation and Transfer

 

Training

Corporate on-site & in-house training

Design, development, and delivery of customized training

 

Technology Diffusion

Rental of Space and Equipment

Industry-specific events & symposia

 

Inventory of Specialized Equipment

  • Analytical chemistry:
    • Mass spectrometer MS MS AB Sciex (3200 Q Trap) Coupled with HPLC-UV/VIS LC Shimadzu (LC-20AD, DGU-20A3R,
    • Mass spectrometer MS Coupled with HPLC-UV/VIS :: LC Shimadzu (LC-20AD, DGU-20A3R, SIL-20ACHT); Separation and quantification of soluble molecules;
    • HPLC-UV/VIS Varian (920-LC)
    • Ion chromatograph Thermo Scientific (Dionex lCS-5000 DC, Dionex lCS-5000 SP Dionex AS-AP)
    • Atomic absorption spectrometer Varian (AA240)
    • GC-MS (with head space) Agilent (6890N/5975C)
    • GC-FID Agilent (7890A)
    • Capillary electrophoresis Agilent (HP 3D CE)
    • Differential scanning calorimeter (DSC) PerkinElmer (JADE)
    • Near-Infrared (FT-NIR) Bruker (TANGO spectrometer)
  • Cell culture BSL2 facilities:
    • Laminar Flow Hoods
    • CO2 incubators
    • Inverted microscopes (Cellular organization and morphology)
  • Fluorescence microscope OLYMPUS (BX53F)Visualization of fluorescent cellular structures and probes;
  • Stereomicroscope OLYMPUS (SZX-16)Cellular organization and morphology (fluorescence capabilities);Light and temperature controlled for cell and plant culture;Growth chamber VWR

 

Elemental analysis:

  • Kjeltec digestor and distillator (N) FOSS (Kjeltec 8000) Quantification of nitrogen in solid and liquid samples;
  • Combustion Dumas (CNS) LECO (TruSpec CN,TruSpec S) Quantification of nitrogen, carbon and sulfur in solid samples;

Genetic suite:

  • PCR BIO-RAD (S1000 Thermal Cycler) DNA amplification
  • PCR DNA Engine (3) MJ Research (PTC 200) DNA amplification
  • Gel imager BIO-RAD (Molecular Imager Gel doc XR +)
     Digitalization of gels;
  • DNA sequencer ABI 3500 DNA sequencing and genotype analysis
  • RT-PCR BIO-RAD CFX 96 DNA amplification in real-time
  • DNA Extraction EZ1 robot Automated DNA extraction

Microbiology BSL2 facilities:

  • Biolog GEN III OmniLog Combo Plus System; Bacterial identification
  • Anaerobic chamber Plas Labs Anaerobic handling
  • Incubator shaker Fisher (MaxQ) Temperature and agitation

Nutritional analysis:

  • Fiber analyzer Ankom (A-200) Determination of neutral detergent fiber and acid detergent fiber in plant material;
  • Soxhlet extraction unit Foss (Soxtec 2045) Lipuid extraction
  • Rancimat Metrohm (892 Rancimat) Determination of lipid oxidation in samples containing oil and fat
  • Water activity meter Neutec (Lab Master.aw) Measurement of water activity in a variety of food samples

Particules analysis:

  • Multisizer Z4 (20, 30, 50, 100 et 2 000 μm aperture) Beckman Coulter (Multisizer 4) Determination of particle size distribution and quantification

Recombinant protein production and purification BSL2 facilities:

  • Bioreactors 1-L (3) Applikon (Z61101C004) Small scale culture of microorganisms (w.v. 0.75 L);
  • Bioreactors 7.5-L (2) Labfors (Infors) Small scale culture of microorganisms (w.v. 5 L);
  • Photobioreactor 1.9-L Lux 5 flat panel (Infors) Production of microalgae (w.v. 1.2 L)
  • Photobioreactor 2-L Labfors 4 + labfors Lux module (Infors) Production of microalgae (w.v. 1.2 L);
  • FPLC BIO-RAD BioLogic Purification of proteins and biologics;

Sample preparation:

  • Grinder Retsch (Zm 200) Grinding, sieving and sizing (0.2, 0.5 and 1 μm);
  • Freeze-dry System (Lyophilizer): Labconco (FreeZone) Freeze-drying of samples;

Spectroscopy/Fluorescence:

  • Spectrophotometer BioTek (SynergyMx) Absorbance/fluorescence/luminescence readings on 96-well plates and cuvette;
  • Spectrophotometer Varian (Eclipse) Fluorescence reading (cuvette based);